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Millipore diphtheria toxin dt
Diphtheria Toxin Dt, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
diphtheria toxin dt - by Bioz Stars, 2026-02
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Drummond Scientific diphtheria toxin (dt
(a) The injections of AAV-sgTrpa1 and AAV-sgScramble into the right nodose ganglion are performed to generate NG Trpa1KO mice and Ctrl mice, respectively. (b) The gating strategy of CGRP + EPCAM + NCAM + PNECs from NG Trpa1KO mice and Ctrl mice. (c) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (d) Comparison of CGRP mean fluorescence intensity in CGRP + EpCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (e) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG Trpa1KO mice and Ctrl mice following LPS stimulation (n = 5 mice/group). (f) <t>Diphtheria</t> Toxin (DT) was injected into the nodose ganglia in Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice. Three weeks after DT injection, a 7-day LPS stimulation model was established. (g and h) During the 7 days of LPS stimulation, NG CalcaABLATE mice exhibited lower levels of TNF-α in BALF compared to Ctrl mice (n = 3 mice/group). (i) The mortality rate in NG CalcaABLATE mice and Ctrl mice during 7 days of LPS stimulation. (j) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (k) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (l and m) The proportion of Ki67 + PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). Student’s t test in (c), (d) and (e). One-way ANOVA in (h), (j), (k) and (m). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.
Diphtheria Toxin (Dt, supplied by Drummond Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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diphtheria toxin (dt - by Bioz Stars, 2026-02
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(a) The injections of AAV-sgTrpa1 and AAV-sgScramble into the right nodose ganglion are performed to generate NG Trpa1KO mice and Ctrl mice, respectively. (b) The gating strategy of CGRP + EPCAM + NCAM + PNECs from NG Trpa1KO mice and Ctrl mice. (c) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (d) Comparison of CGRP mean fluorescence intensity in CGRP + EpCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (e) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG Trpa1KO mice and Ctrl mice following LPS stimulation (n = 5 mice/group). (f) Diphtheria Toxin (DT) was injected into the nodose ganglia in Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice. Three weeks after DT injection, a 7-day LPS stimulation model was established. (g and h) During the 7 days of LPS stimulation, NG CalcaABLATE mice exhibited lower levels of TNF-α in BALF compared to Ctrl mice (n = 3 mice/group). (i) The mortality rate in NG CalcaABLATE mice and Ctrl mice during 7 days of LPS stimulation. (j) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (k) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (l and m) The proportion of Ki67 + PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). Student’s t test in (c), (d) and (e). One-way ANOVA in (h), (j), (k) and (m). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.

Journal: bioRxiv

Article Title: Neural circuits between nodose ganglion and pulmonary neuroendocrine cells regulate lung inflammatory responses

doi: 10.1101/2025.01.01.630820

Figure Lengend Snippet: (a) The injections of AAV-sgTrpa1 and AAV-sgScramble into the right nodose ganglion are performed to generate NG Trpa1KO mice and Ctrl mice, respectively. (b) The gating strategy of CGRP + EPCAM + NCAM + PNECs from NG Trpa1KO mice and Ctrl mice. (c) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (d) Comparison of CGRP mean fluorescence intensity in CGRP + EpCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (e) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG Trpa1KO mice and Ctrl mice following LPS stimulation (n = 5 mice/group). (f) Diphtheria Toxin (DT) was injected into the nodose ganglia in Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice. Three weeks after DT injection, a 7-day LPS stimulation model was established. (g and h) During the 7 days of LPS stimulation, NG CalcaABLATE mice exhibited lower levels of TNF-α in BALF compared to Ctrl mice (n = 3 mice/group). (i) The mortality rate in NG CalcaABLATE mice and Ctrl mice during 7 days of LPS stimulation. (j) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (k) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (l and m) The proportion of Ki67 + PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). Student’s t test in (c), (d) and (e). One-way ANOVA in (h), (j), (k) and (m). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Then, 20 ng of diphtheria toxin (DT) in 120 nL of PBS was injected into the NG using a nanoinjector (Drummond Scientific Company).

Techniques: Expressing, Comparison, Fluorescence, Injection

(a) Diphtheria Toxin (DT) was injected into the nodose ganglia in Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice. (b and c) CGRP immunostaining (green) in the nodose/jugular complex (B) and lung (C) of Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice following NG injection, with DAPI nuclear counterstaining. (d-e) The gating strategy of CGRP + EPCAM + NCAM + PNECs from NG CalcaABLATE mice and Ctrl mice following LPS stimulation (D). The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation (E). (n = 3-7 mice/group) (f) Comparison of CGRP mean fluorescence intensity in CGRP + EpCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation. (n = 3-7 mice/group) (g) The proportion of CGRP + EPCAM + NCAM + cells among all CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation. (n = 3-7 mice/group) (h) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation. (n = 3-7 mice/group) (i) LPS induced a lower level of TNFα in F4/80 + macrophages in the BALF of NG CalcaABLATE mice compared with Ctrl mice (n = 3-7 mice/group). Scale bars: 100 μm in (c). 50 μm in (b). One-way ANOVA in (e), (f), (g), (h) and (i). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.

Journal: bioRxiv

Article Title: Neural circuits between nodose ganglion and pulmonary neuroendocrine cells regulate lung inflammatory responses

doi: 10.1101/2025.01.01.630820

Figure Lengend Snippet: (a) Diphtheria Toxin (DT) was injected into the nodose ganglia in Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice. (b and c) CGRP immunostaining (green) in the nodose/jugular complex (B) and lung (C) of Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice following NG injection, with DAPI nuclear counterstaining. (d-e) The gating strategy of CGRP + EPCAM + NCAM + PNECs from NG CalcaABLATE mice and Ctrl mice following LPS stimulation (D). The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation (E). (n = 3-7 mice/group) (f) Comparison of CGRP mean fluorescence intensity in CGRP + EpCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation. (n = 3-7 mice/group) (g) The proportion of CGRP + EPCAM + NCAM + cells among all CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation. (n = 3-7 mice/group) (h) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following LPS stimulation. (n = 3-7 mice/group) (i) LPS induced a lower level of TNFα in F4/80 + macrophages in the BALF of NG CalcaABLATE mice compared with Ctrl mice (n = 3-7 mice/group). Scale bars: 100 μm in (c). 50 μm in (b). One-way ANOVA in (e), (f), (g), (h) and (i). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Then, 20 ng of diphtheria toxin (DT) in 120 nL of PBS was injected into the NG using a nanoinjector (Drummond Scientific Company).

Techniques: Injection, Immunostaining, Expressing, Comparison, Fluorescence

(a) Sequential intraperitoneal injections of tamoxifen and diphtheria toxin were used to genetically ablate PNECs in Ascl1CreERT2; loxP-DTR ( Ascl1 ABLATE ) mice and loxP-DTR ( Ctrl ) mice. (b and c) The proportion of EPCAM + NCAM + cells among CD45-cells was assessed in Ascl1 ABLATE mice and Ctrl mice. (n = 5 mice/group). (d) Vagal responses to LPS stimuli within the lung in Ascl1 ABLATE mice and Ctrl mice. (n = 3-7 mice/group) (e) Quantification of maximum responses to LPS in Trpa1KO mice and Ctrl mice (n = 3 mice/group). (f) Following LPS stimulation, the level of TNF-α in the BALF of Ascl1 ABLATE mice was lower compared to that in Ctrl mice (n =4-5 mice/group). (g and h) Following LPS stimulation, Ascl1 ABLATE mice exhibited a lower level of TNF-α in F4/80 + macrophages in the BALF compared to Ctrl mice (n = 4-5 mice/group). (i) The proportion of Ly6G high Ly6C low cells among CD11B + cells in lung tissue was assessed in Ascl1 ABLATE mice and Ctrl mice following LPS stimulation (n = 4-5 mice/group). (j) The proportion of Ly6G high Ly6C low CD11b + cells (PMNs) among live cells in lung tissue was assessed in Ascl1 ABLATE mice and Ctrl mice following LPS stimulation (n = 4 mice/group). (k) Il6 and Ccl2 expression levels in lung tissue were assessed in Ascl1 ABLATE mice and Ctrl mice following LPS stimulation. (n = 6-10 mice/group) (l) The mortality rate in Ascl1 ABLATE mice and Ctrl mice during 7 days of LPS stimulation. Scale bars:100 μm in (a). Student’s t test in (c) and (e).One-way ANOVA in (f), (h), (j) and (k). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.

Journal: bioRxiv

Article Title: Neural circuits between nodose ganglion and pulmonary neuroendocrine cells regulate lung inflammatory responses

doi: 10.1101/2025.01.01.630820

Figure Lengend Snippet: (a) Sequential intraperitoneal injections of tamoxifen and diphtheria toxin were used to genetically ablate PNECs in Ascl1CreERT2; loxP-DTR ( Ascl1 ABLATE ) mice and loxP-DTR ( Ctrl ) mice. (b and c) The proportion of EPCAM + NCAM + cells among CD45-cells was assessed in Ascl1 ABLATE mice and Ctrl mice. (n = 5 mice/group). (d) Vagal responses to LPS stimuli within the lung in Ascl1 ABLATE mice and Ctrl mice. (n = 3-7 mice/group) (e) Quantification of maximum responses to LPS in Trpa1KO mice and Ctrl mice (n = 3 mice/group). (f) Following LPS stimulation, the level of TNF-α in the BALF of Ascl1 ABLATE mice was lower compared to that in Ctrl mice (n =4-5 mice/group). (g and h) Following LPS stimulation, Ascl1 ABLATE mice exhibited a lower level of TNF-α in F4/80 + macrophages in the BALF compared to Ctrl mice (n = 4-5 mice/group). (i) The proportion of Ly6G high Ly6C low cells among CD11B + cells in lung tissue was assessed in Ascl1 ABLATE mice and Ctrl mice following LPS stimulation (n = 4-5 mice/group). (j) The proportion of Ly6G high Ly6C low CD11b + cells (PMNs) among live cells in lung tissue was assessed in Ascl1 ABLATE mice and Ctrl mice following LPS stimulation (n = 4 mice/group). (k) Il6 and Ccl2 expression levels in lung tissue were assessed in Ascl1 ABLATE mice and Ctrl mice following LPS stimulation. (n = 6-10 mice/group) (l) The mortality rate in Ascl1 ABLATE mice and Ctrl mice during 7 days of LPS stimulation. Scale bars:100 μm in (a). Student’s t test in (c) and (e).One-way ANOVA in (f), (h), (j) and (k). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.

Article Snippet: Then, 20 ng of diphtheria toxin (DT) in 120 nL of PBS was injected into the NG using a nanoinjector (Drummond Scientific Company).

Techniques: Expressing