Journal: bioRxiv
Article Title: Neural circuits between nodose ganglion and pulmonary neuroendocrine cells regulate lung inflammatory responses
doi: 10.1101/2025.01.01.630820
Figure Lengend Snippet: (a) The injections of AAV-sgTrpa1 and AAV-sgScramble into the right nodose ganglion are performed to generate NG Trpa1KO mice and Ctrl mice, respectively. (b) The gating strategy of CGRP + EPCAM + NCAM + PNECs from NG Trpa1KO mice and Ctrl mice. (c) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (d) Comparison of CGRP mean fluorescence intensity in CGRP + EpCAM + NCAM + cells in lung tissue from NG Trpa1KO mice and Ctrl mice (n = 5 mice/group). (e) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG Trpa1KO mice and Ctrl mice following LPS stimulation (n = 5 mice/group). (f) Diphtheria Toxin (DT) was injected into the nodose ganglia in Calca CreERT2 ; loxP-DTR ( NG CalcaABLATE ) mice and loxP-DTR ( Ctrl ) mice. Three weeks after DT injection, a 7-day LPS stimulation model was established. (g and h) During the 7 days of LPS stimulation, NG CalcaABLATE mice exhibited lower levels of TNF-α in BALF compared to Ctrl mice (n = 3 mice/group). (i) The mortality rate in NG CalcaABLATE mice and Ctrl mice during 7 days of LPS stimulation. (j) The proportion of highly CGRP-expressing PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (k) The number of PNECs per 5*10 5 CD45 − cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). (l and m) The proportion of Ki67 + PNECs among all CGRP + EPCAM + NCAM + cells in lung tissue from NG CalcaABLATE mice and Ctrl mice following 7 days of LPS stimulation (n = 3-4 mice/group). Student’s t test in (c), (d) and (e). One-way ANOVA in (h), (j), (k) and (m). Mean ± SEM. ns, not significant. *p < 0.05, **p < 0.01, and ***p < 0.001.
Article Snippet: Then, 20 ng of diphtheria toxin (DT) in 120 nL of PBS was injected into the NG using a nanoinjector (Drummond Scientific Company).
Techniques: Expressing, Comparison, Fluorescence, Injection